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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but specific nucleotide sequence details will not be nonetheless offered.
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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide generally has the epitope recognized with the antibody.
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This manual outlines additional feasible optimization of mobile and immunoassay parameters to be certain the absolute best benefits are...
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This manual outlines even more attainable optimization of cellular and immunoassay parameters to be sure the very best benefits are...
Antibodies sure to the blocking peptide no more bind to your epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinct expression and degradation designs which lead towards the temporal coordination of each mitotic celebration. Cyclin E varieties a posh with and capabilities to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells progress via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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